Research Director, Biyani Group of Colleges (http://www.biyanicolleges.org) R-4, Sector 3, Vidhyadhar Nagar, Jaipur, India Tel. +91-141-233-8371, Fax +91-141-233-6226 E-mail: director@biyanicolleges.org; biyanijp@yahoo.co.in Webpage: www.drmanishbiyani.org

Chief Scientist, University of Tokyo (http://bionano.t.u-tokyo.ac.jp) NanoBio Device Lab, Dept of Bioengineering 2-11-16 Yayoi, Bunkyo-ku, Tokyo, Japan Overseas Tel/Fax +81-3-5841-1180 E-mail: biyani@bionano.t.u-tokyo.ac.jp

Born Oct. 1, 1973 in Jaipur, India and married Nov. 21, 2000 with Dr. Madhu Maheshwari.

March, 2004 Ph.D. in Biochemistry and Biophysics with a concentration in molecular structural biology from Saitama University, JAPAN. Thesis work with Prof. Koichi Nishigaki and Prof. Yuzuru Husimi entitled “Study on Structural Dynamics of ssDNA performed by Molecular Dynamics and Gel Electrophoresis”.
March, 2001 M.Sc. (Master Degree in Genetics Engineering) with a concentration in Biotechnology from Saitama University, JAPAN. Thesis work with Prof. Koichi Nishigaki entitled "Micronization and exploitation of TGGE technology for the study of solution structures of DNAs”.
July, 1998 Graduation in Pharmacy with a concentration in Medicine from University of Rajasthan, INDIA.
July, 1994 B.Sc. (Bachelor Degree in Science) with a concentration in Biology from University of Rajasthan, INDIA.
Professional Career:

Oct, 2008 Group leader in CREST Project, University of Tokyo, Japan
Oct, 2007 Research Scientist in University of Tokyo, Center for NanoBio Integration, Japan
May, 2006 Research Director and Visiting Faculty member, BISMA (Biyani Institute of Science and Management), Biyani Girls College, India
April, 2004 Group Leader & Research Scientist in REDS Project (¥ 250 millions) (Rational Evolutionary Design of Advanced Biomolecules, Saitama), CREATE, JST, Japan.
Aug, 2001 Visiting Researcher, Washington University, USA
Professional Scientific Services:

• • Executive member & Chapter President, ISAJ, Indian Scientist Association in Japan, (2008 ~ present)
  • Chair and Chief committee member, BISMA-Biotech Conference (2006 ~ present)
  • Ad hoc reviewer, Biophysical Journal, HighWire Press (2005 ~ present)
  • Ad hoc reviewer, Analytical Biochemistry, Academic Press (2005 ~ present)
  • Ad hoc reviewer, Electrophoresis, Wiley Press (2005 ~ present)
  • Ad hoc reviewer, Biopolymer, Wiley Press (2007 ~ present)
  • Ad hoc reviewer, Nucleic Acids Res., Oxford Press (2008 ~ present)
  • Ad hoc reviewer, Chemistry Letters, CSJ Press (2008 ~ present)

Publications:
(A) Patents:
1. Miniaturized Micro-Gel Electrophoresis. Japanese National Invention Patent Number(s): JP11-352680, (issued April 2000).
2. Linker-DNA for mRNA-Puromycin-Protein conjugate. Japanese National Invention Patent Number(s): WO2006041194-A1; EP1816192-A1; JP2006541001-X (filed Nov 2, 2004).
3. Solid-phase cell-free Protein synthesis for folding quality control. Japanese National Invention Patent Number(s): WO2006052016-A1; JP2006545148-X; US2008214783-A1 (filed Nov 12, 2004).
4. Microarray reactor chip for high throughput enzymatic assay. Japanese National Invention Patent Number(s): JP2008116218-A (filed Oct 18, 2005).
5. Single clone-type DNA-to-Protein chip for high throughput functional proteomics. Japanese National Invention Patent Number(s): W02008053598-A1 (filed Nov 1, 2006).

• 
  (B) Journals (Refereed papers in International Journals):
  1. Manish Biyani and Koichi Nishigaki (2001) Hundred-fold Productivity of Genome Analysis by Introduction of Micro-Temperature Gradient Gel Electrophoresis. Electrophoresis 22(1), 23-28.
  2. Manish Biyani, Hiroshi Nishizawa, Yoshihide Miyatani, Eiichi Kanaumi, Pharhad Eli, Yasunori Kinoshita, and Koichi Nishigaki (2001) Micronized Gel Electrophoresis: Construction and Its Versatile Applications. Chemistry letters, 2, 138-139.
  3. Manish Biyani and Koichi Nishigaki (2003) Sequence-specific and non-specific mobilities of single-stranded oligonucleotides observed by changing the borate buffer concentration. Electrophoresis, 24(4), 628-633.
  4. Manish Biyani and Koichi Nishigaki (2005) Single-strand conformation polymorphism at the oligodeoxyribonucleotide level: An insight into solution structural dynamics of DNAs by gel electrophoresis and molecular dynamics simulations. J Biochem 138(4), 363-373.
  5. Manish Biyani and Koichi Nishigaki (2005) Structural characterization of ultra-stable higher-ordered aggregates generated by novel guanine-rich DNA sequences. Gene 364, 130-138.
  6. Manish Biyani, Naoto Nemoto and Yuzuru Husimi (2006) Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of protein using anchored mRNA. Nucleic Acids Res. 34, e140.
  7. Manish Biyani, Terutsune Osawa, Naoto Nemoto and Takanori Ichiki (2008) A single molecule-derived DNA-linked protein microarray: a molecular lithographic interface for a self-assembling and self-addressable protein biochip. Lab on a Chip. (under consideration).
  8. Junichi Yamaguchi, Manish Biyani, Yuzuru Husimi, Takashi Funatsu and Naoto Nemoto (2008) cDNA display: A novel method for rapid stabilization of mRNA-protein fusion and its application to the screening of functional proteins. Nature Methods (under consideration)
  9. Manish Biyani, Yoshihiro Kikkawa, Masahiro Fujita and Koichi Nishigaki (2007) DNA directed multidimensional self-assembly growth for nanoarchitectures: model and evidences. Nature Materials (under construction)
  10. Manish Biyani, Madhu Biyani and Naoto Nemoto (2008) UTR Paper. BBRC (under construction)
• 
  (C) Books and Refereed papers in International Conferences:
  11. Manish Biyani and Koichi Nishigaki. (2003) Prediction from Sequence to Structure at oligonucleotides level by MD simulations. In: Proc. of 14th International conference on Genome Informatics 2003, Gribskov, M., Kanehisa, M., Miyano, S., Takagi, T. (ed.), Universal academy press, Japan, vol.14, p.p.490-491.
  12. Yosuke Hosoi, Manish Biyani, Naoto Nemoto and Takanori Ichiki (2005) High-Throughput Screening of mutant AKR enzymes using mRNA display and novel microreactor array chips. In: Proc. of 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (MicroTAS2005), Boston, USA, vol.1, p.p. 323-325.
  13. Manish Biyani and Naoto Nemoto (2005) An artificial co-evolutionary relationship between ribsome/mRNA interaction (translational initiation) and protein synthesis driven by in-vitro-virus. In: Proc. of Hayashibara Forum 2005, Okayama, Japan, vol.??, p.p??.
  14. Yosuke Hosoi, K. Takahashi, Manish Biyani, Naoto Nemoto, Takanori Akagi and Takanori Ichiki (2005) High-throughput screening of mutant biomolecules using mRNA display and microreactor array chips. In: Proc. of International Conference on Solid State Device and Materials (SSDM 2005), Kobe, Japan, pp.452-453.
  15. Manish Biyani, Keiichi Hamano, Yasushi Hirano, Motofumi Suzuki and Koichi Nishigaki (2006) Genome Profiling (GP): a universal method for inter- and intra-species identification. In: Proc. of 93rd Indian Science Congress, Mukherjee S.P. (ed.), Hyderabad, India, vol., p.p. 81.
  16. Manish Biyani, Naoto Nemoto, Yosuke Hosoi and Takanori Ichiki (2006) RNA-to-Protein Chip: an integrated microarray platform to bridge genomics and proteomics. In: Proc. of 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (MicroTAS2006), Tokyo, Japan, vol.1, p.p.209-211.
  17. Manish Biyani (2006) State-of-the-art Genome Profiling (GP): a key tool for plant biotechnology. In: Recent advancement in Plant Biotechnology. Kumar A., Sopory S. (ed), IK International publisher, New Delhi, pp 70-78.
  18. Manish Biyani, Terutsune Osawa, Naoto Nemoto and Takanori Ichiki (2008) One-to-one gene-encoded functional protein microarray. In: Proc. of 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences. (MicroTAS2008), San diego, USA, vol.2, pp 1781-1783.
  Awards & Honors:

• Sept 2008: Invited and appointed as Referee to review scientific manuscript submitted from Saitama University (Japan) to Chemistry Letters international journal (CSJ Press).
  July 2008: Travel grant fellowship from Department of Science and Technology, Government of India to participate in the XX International Congress of Genetics in Berlin, Germany.
  March 2008: Invited and appointed as Referee to review scientific manuscript submitted from Osaka University (Japan) to Nucleic Acids Res. international journal (Oxford Press).
  Jan 2008: Invited as chairperson and speaker in 8th International symposium on Biomimetic Materials Processing, Nagoya, Japan.
  Dec 2007: Invited as speaker in 18th Materials Research Society Symposium, Tokyo, Japan.
  June 2007: Invited and appointed as Referee to review scientific manuscript submitted from Georgia State University (USA) to Biopolymer international journal (Wiley Press).
  April 2007: Invited speaker in Cambridge Health tech Institute’s seven annual conference on Genomic and Proteomic sample preparation and third annual G.O.T. Summit, Boston, USA.
  March 2007: Selected and invited speaker by Biophysical Society, USA for presenting a talk in 51st Annual meeting in Baltimore, USA.
  Nov 2006: Honored by Glen Research Corporation to include in Glen Research Literature Highlights archive.
  June 2006: JB OUP Prize for the best poster presentation of exceptional value at the 10th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress.
  Jan 2006: Invited speaker in 93rd Indian Science Congress (ISC2006), Hyderabad, India.
  Dec 2005: Invited speaker in International symposium on Strategies of Life: a self-sustaining network of molecular information, Hayashibara Forum 2005, Okayama, Japan.
  Dec 2005: Selected speaker by Molecular Biology Society, Japan for presenting a talk in 28th Annual Meeting in Fukuoka, Japan.
  July 2005: Invited and appointed as Referee to review scientific manuscript submitted from Germany to Electrophoresis international journal (Wiley Publisher).
  June 2005: Invited and appointed as Referee to review scientific manuscript submitted from Iceland to Analytical Biochemistry international journal (Academic Press).
  April 2005: Invited as Referee to review scientific manuscript submitted from California to Biophysical Journal (HighWire Press).
  Sept 2004: Two of published papers in International Journal (Electrophoresis 2003 and Chemistry Letter 2001) have been selected to abstracted in Analytical WebBase from The Royal Society of Chemistry (RSC), the largest organization in Europe for advancing the chemical sciences.
  Oct 1998 to March 2004: Awarded for Japanese Govt. Scholarship (Monbusho) from Ministry of Education, Japan.
  July 2003: Bursary from International Genetics Federation to participate in the XIX International Congress of Genetics in Melbourne, Australia.
  Jan 2001: Pioneered techniques for the fabrication of miniaturized version of Gel Electrophoresis (µGE, Micro Gel Electrophoresis; µTGGE, Micro Temperature Gradient Gel Electrophoresis; mmTGGE, multi micro Temperature Gradient Gel Electrophoresis) with 100-fold or more productivity of Genome analysis.
  Aug 2000: Invited as a visiting scientist to attend a seminar in Washington University, School of Medicine, St. Louis, USA.
  July 1998: Outstanding academic award for getting first rank during Pharmacy course from pharmacy medical association of Rajasthan, India.

Invited and Participated Public Talks
(A) International conferences:
1. 12th International conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS2008), San Diego, USA (Oct 2008). One-to-one gene-encoded functional protein microarray. (Poster)
2. 3rd Annual international symposium on Facilitation interdisciplinary Biotech for future entrepreneurship (BISMA-Biotech 2008), Jaipur, INDIA (Aug 2008). Interdisciplinary Biotech (IDB): an idea whose time has come. (Invited Opening lecture, Chair)
3. XX International Congress of Genetics, Berlin, GERMANY (July 2008) State-of-the-art Genome Profiling: technological advances for species-rich ‘microbiome’. (Poster)
4. 8th International symposium on Biomimetic Materials Processing (BMMP-8), Nagoya, JAPAN (Jan 2008). Molecular Biomimetic approaches for ‘next generation’ protein microarray. (Invited Oral, Chair)
5. 18th Materials Research Society Symposium (MRS-J), Tokyo, JAPAN (Dec 2007). On-chip Protein factory: state-of-the-art protein-nanomaterial interface for next generation protein-bio chip. (Invited Oral)
6. International symposium on Advanced Functional Genomics, Kazusa, JAPAN (Oct 2007). One-spot-one-gene expression and functional assay type microarrays for ‘next-generation’ genomics. (Poster)
7. 2ndAnnual international seminar on Biotechnology Education (BISMA-Biotech 2007), Jaipur, INDIA (June 2007). On-chip protein factory: solution to the future challenges. (Invited Oral, Chairperson)
8. GOT Summit 2007, Boston, USA (April 2007). Preparing and Handling Templates for High-Throughput Functional Genomics: Approaches and Challenges (Invited Oral)
9. 51st Biophysical Annual meeting, Baltimore, USA (March 2007). DNA-to-Protein Chip: a microarray platform for genotype-phenotype linkage-based, single-gene-encoded protein biochip. (Oral)
10. 51st Biophysical Annual meeting, Baltimore, USA (March 2007). Improvement of protein foldability by solid-phase cell-free translation. (Poster)
11. 10th International conference on Miniaturized Systems for Chemistry and Life Science (MicroTAS2006), Tokyo, JAPAN (Nov 2006). RNA-to-Protein Chip: more specific and integrated microarray platform to bridge genomics and proteomics. (Poster)
12. 5th East Asia Biophysics Symposium & 44th Annual meeting of the Biophysical Society of Japan (EABS & BSJ 2006), Okinawa, JAPAN (Nov 2006). Directed evolution of IRES for promoting cap-independent cell-free Translation system. (Poster)
13. 5th East Asia Biophysics Symposium & 44th Annual meeting of the Biophysical Society of Japan (EABS & BSJ 2006), Okinawa, JAPAN (Nov 2006). On-chip Evolution: Overcoming of fluctuation in analysis of a single molecule activity in a picoliter well. (Poster)
14. 1st Annual international symposium on Research-based Education for Hi-biotech innovation (Biotech-BISMA 2006), Jaipur, INDIA (June 2006). Nano-Bio-Info technologies: Shaping big future on a small scale. (Invited Oral)
15. XX International Congress of Biochemistry and Molecular Biology (IUBMB2006), Kyoto, JAPAN (June 2006). On-chip Evolution: Development of novel bio-reactor microarray chip for genotype-phenotype linking technology. (Poster)
16. XX International Congress of Biochemistry and Molecular Biology (IUBMB2006), Kyoto, JAPAN (June 2006). Control of cell-free translation initiation by evolving a universal regulatory sequence using genotype-phenotype linking technology. (Poster)
17. 93rd Indian Science Congress (ISC2006), Hyderabad, INDIA (Jan 2006). Genome Profiling (GP): a universal method for inter- and intra-species identification. (Invited Oral)
18. International symposium on Strategies of Life: a self-sustaining network of molecular information, Hayashibara Forum 2005, Okayama, JAPAN (Dec 2005). An artificial co-evolutionary relationship between ribsome/mRNA interaction (translational initiation) and protein synthesis driven by in-vitro-virus. (Invited Oral)
19. Micro Total Analysis Systems (?TAS2005), Boston, USA (Oct 2005). High-Throughput Screening of mutant AKR enzymes using mRNA display and novel microreactor array chips. (Poster)
20. XV International Biophysics Congress (IUPAB 2005), Montpellier, FRANCE (Aug 2005) G-lego: a novel entry of ultra-stable higher ordered aggregates generated by self-assembling of Guanine-rich oligonucleotides. (Poster)
21. XV International Biophysics Congress (IUPAB 2005), Montpellier, FRANCE (Aug 2005) Unstable structures working: Evidences. (Poster)
22. XIV International workshop on Beyond the Identification of Transcribed Sequences (BITS2004), Chiba, JAPAN (Oct 2004) Structural studies of ultra-stable higher ordered aggregates generated by G (guanine)-rich sequences. (Poster)
23. XIV International conference on Genome Informatics (GIW2003), Yokohama, JAPAN (Dec 2003) Prediction from Sequence to Structure at oligonucleotides level by MD simulations. (Poster)
24. XIX International Congress of Genetics, Melbourne, AUSTRALIA (July 2003) Characteristic solution structures of oligonucleotides revealed by molecular dynamics and gel electrophoresis. (Poster)
25. Human Genome Meeting (HGM2002), Shanghai, CHINA (April 2002) Sequence-specific mobilities of single-stranded oligonucleotides in polyacrylamide gel electrophoresis inferring conformation dynamics. (Poster)
26. International Conference in Bio Physics, Kyoto, JAPAN (July 2001) Structure–mobility correlation of ssDNAs investigated by micro-electrophoresis and molecular dynamics. (Oral)

• (B) National conferences:
  27. National symposium on Biotechnology in 21st Century: The challenges ahead for new advances towards food security, Jaipur, INDIA (Dec 2005) New Biotechnologies for Genome analysis and evolution. (Oral)
  28. 28th Annual Meeting of the Molecular Biology Society of Japan, Fukuoka, JAPAN (Dec 2005) Searching a new regulatory sequence for efficient initiation of cell-free translation from a synthetic random library by in vitro virus. (Oral)
  29. 28th Annual Meeting of the Molecular Biology Society of Japan, Fukuoka, JAPAN (Dec 2005). (Poster)
  30. 43th Japanese Biophysics Conference, Sapporo, JAPAN (Nov 2005) Genotype-phenotype linked Microarray Evolution Reactor: Construction and screening a new fluorescent protein from random-sequence space. (Poster)
  31. 43th Japanese Biophysics Conference, Sapporo, JAPAN (Nov 2005) An “Artificial selection”-type Evolution Reactor machine for searching high-affinity molecules from random sequences by In-vitro-virus. (Poster)
  32. 43th Japanese Biophysics Conference, Sapporo, JAPAN (Nov 2005) High-throughput screening of mutant AKR enzymes using genotype-phenotype linked microarray evolution reactor chip. (Poster)
  33. 59th conference of the Science on Form, Sapporo, JAPAN (June 2005) Unstable structures of oligopeptides/oligonucleotides and the significance of the study on those structures. (Poster)
  34. 27th Annual Meeting of the Molecular Biology Society of Japan, Kobe, JAPAN (Dec 2004) Protein synthesis using immobilized mRNA by in vitro virus method. (Poster)
  35. 42nd Japanese Biophysics Conference, Kyoto, JAPAN (Dec 2004) Protein synthesis on bead using in vitro virus: Can immobilized mRNA enhance the translation and folding of protein? (Poster)
  36. 40th Japanese Biophysics Conference, Nagoya, JAPAN (Nov 2002) Solution structure dynamics of single-stranded oligonucleotides: Experiments and molecular dynamics. (Oral)
  37. 38th Japanese Biophysics Conference, Sendai, JAPAN (Sept 2000) Micro-TGGE: a simple, rapid and cost/time-saving method for Genome analysis. (Oral)
  38. 37th Japanese Biophysics Conference, RIKEN, JAPAN (Sept 1999) Study on the correlation of structure and mobility of DNAs with coordinated use of DNA-melting-simulation and TGGE. (Poster)
  Ongoing Research Projects:
  1. A novel solid-phase cell-free Translation and RNA-protein fusion
  2. DNA-to-Protein chip: an HTP microarray platform to bridge genomics and proteomics
  3. Oligo-origami: DNA directed multi-dimensional self-assembly growth for nanoarchitectures
  4. µGE/µTGGE: Micronization of gel electrophoresis with 100-fold productivity
  5. Genome Profiling (GP): a universal method for inter- and intra-species identification
  6. SSCP: Structural dynamics of single-stranded DNA/RNA at the oligonucleotide level
  Membership of Scientific Societies:
  1. Biophysical Society of America.
  2. Biophysical Society of Japan.
  3. Molecular Biology Society of Japan.
  4. The Indian Science Congress Association.
  Research Collaborators:
  1. Saitama University (Saitama, JAPAN): Prof. Koichi NISHIGAKI, Prof. Yuzuru HUSIMI
  2. The University of Tokyo (Tokyo, JAPAN): Prof. Takanori ICHIKI
  3. RIKEN (Wako, JAPAN): Dr. Masahiro FUJITA
  4. RIKEN (Yokohama, JAPAN): Prof. Yoshifumi NISHIMURA
  5. Shimadzu (Kyoto, JAPAN): Dr. Masamitsu SHIKATA
  6. Janusys (Saitama, JAPAN): Dr. Naoto NEMOTO
  7. Taitec (Saitama, JAPAN): Dr. Keiichi HAMANO
  8. The University of Rajasthan (Jaipur, INDIA): Prof. Ashwani KUMAR
  9. The University of Delhi (Delhi, INDIA): Prof. Rup LAL
  10. ITRC (Industrial Toxicology Research Center, Lucknow, INDIA): Dr. Ashwani KUMAR